The AlphaLISA® Somatostatin Detection Kit is designed for detection and quantitation of somatostatin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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In an AlphaLISA competition assay, a Biotinylated analog of the analyte of interest, the tracer, binds to the Streptavidin-coated Alpha Donor beads, while the Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of low analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. In the presence of high analyte, the beads are separated resulting in lower emission.
Somatostatin is a short peptide hormone (14 or 28 amino acids) involved in regulating the endocrine system. This kit detects both active forms of peptide (Somatostatin-14 and Somatostatin-28). It plays a role in neurotransmission and cell proliferation. Somatostatin interacts with the G protein-coupled Somatostatin receptor to inhibit insulin and glucagon secretion. This peptide exists in almost all vertebrates; coded by six different genes (SS1-6). Classified as an inhibitory hormone, Somatostatin inhibits the release of growth hormone and thyroid-stimulating hormone in the pituitary and multiple gastrointestinal hormones: gastrin, CCK, secretin, and GIP.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Hormone, Peptide|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Quantity in a Package Amount||1.0 Units|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Central Nervous System|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Somatostatin AL329||PDF 880 KB|