The AlphaLISA® Human Soluble Amyloid Precursor Protein alpha (sAPPα) (C-term specific) Detection Kit is designed for detection and quantitation of human sAPPα in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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One antibody targets a region in the N-terminal region of the protein: mouse monoclonal antibody, clone number OM84. The second antibody is specific to the a-secretase cleavage site at the C-terminus: mouse monoclonal antibody, clone number 2B3.
Soluble Amyloid Precursor Protein alpha (sAPPa) is a 670 amino acid protein resulting from cleavage of a cell surface receptor (APP, Amyloid Precursor Protein) by a-secretase. APP is a ubiquitous transmembrane glycoprotein and is also the source of beta-amyloid peptides. The soluble sAPPa is found in cerebrospinal fluid and has been implicated in various cellular processes including neuroprotection. Also, sAPPa may serve a memory-enhancing function, as it regulates neuronal excitability and synaptic plasticity, especially in neurodegenerative diseases such as Alzheimer’s disease.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|One Unit Contains||5000 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Central Nervous System|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA human sAPPa AL254||PDF 236 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|