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sAPPα (C-term specific) (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Soluble Amyloid Precursor Protein alpha (sAPPα) (C-term specific) Detection Kit is designed for detection and quantitation of human sAPPα in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number List Price Your Price Quantity
AL254F 9500.00 USD

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One antibody targets a region in the N-terminal region of the protein: mouse monoclonal antibody, clone number OM84. The second antibody is specific to the a-secretase cleavage site at the C-terminus: mouse monoclonal antibody, clone number 2B3.


  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Soluble Amyloid Precursor Protein alpha (sAPPa) is a 670 amino acid protein resulting from cleavage of a cell surface receptor (APP, Amyloid Precursor Protein) by a-secretase. APP is a ubiquitous transmembrane glycoprotein and is also the source of beta-amyloid peptides. The soluble sAPPa is found in cerebrospinal fluid and has been implicated in various cellular processes including neuroprotection. Also, sAPPa may serve a memory-enhancing function, as it regulates neuronal excitability and synaptic plasticity, especially in neurodegenerative diseases such as Alzheimer’s disease.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target sAPPα
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 5000 assay points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Central Nervous System
Unit Size 5,000 assay points

Resource Library & Events

Resource Type File Name File Format
Data Sheet Manual AlphaLISA human sAPPa AL254 PDF 236 KB
Poster AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices PDF 273 KB