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Prolactin (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Prolactin Detection Kit is designed for detection and quantitation of human Prolactin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number Unit Size List Price Your Price Quantity
AL227C 500 assay points 1433.00 USD
AL227F 5,000 assay points 9500.00 USD

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Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Prolactin (PRL) is an endocrine factor primarily synthesized in the lactotrophs of the anterior pituitary but its production has also been proved in placenta, mammary epithelium and cancers, spleen, sweat gland, bone marrow, thymus, hypothalamus, skin fibroblasts and lymphocytes. PRL has several functions and autocrine and paracrine mechanisms have been shown. It stimulates growth, development and differentiation of breast epithelial cells, and promotes and maintains lactation during pregnancy and suckling. It also inhibits lipopropetin lipase activity in adipose tissue, shows angiogenic effects, and plays a role in the proliferation and differentiation of lymphocytes. The main form of human PRL is a 22 kDa globular protein (199 amino acids), but post-transcriptional and post-translational modifications such as alternative splicing, glycosylation, and proteolytic cleavage have been reported, leading to several forms from 14 to 23 kDa. PRL exerts its function through binding to the PRL Receptor (PRLR) presents in numerous tissues.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Prolactin
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Metabolic
Unit Size 500 assay points