The AlphaLISA® Human Prolactin Detection Kit is designed for detection and quantitation of human Prolactin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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Prolactin (PRL) is an endocrine factor primarily synthesized in the lactotrophs of the anterior pituitary but its production has also been proved in placenta, mammary epithelium and cancers, spleen, sweat gland, bone marrow, thymus, hypothalamus, skin fibroblasts and lymphocytes. PRL has several functions and autocrine and paracrine mechanisms have been shown. It stimulates growth, development and differentiation of breast epithelial cells, and promotes and maintains lactation during pregnancy and suckling. It also inhibits lipopropetin lipase activity in adipose tissue, shows angiogenic effects, and plays a role in the proliferation and differentiation of lymphocytes. The main form of human PRL is a 22 kDa globular protein (199 amino acids), but post-transcriptional and post-translational modifications such as alternative splicing, glycosylation, and proteolytic cleavage have been reported, leading to several forms from 14 to 23 kDa. PRL exerts its function through binding to the PRL Receptor (PRLR) presents in numerous tissues.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Hormone|
|Experimental Type||In vitro|
|One Unit Contains||5000 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Human Prolactin AL227||PDF 204 KB|
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