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Progesterone AlphaLISA Detection Kit, 5,000 assay points

The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number List Price Your Price Quantity
AL335F 9500.00 USD

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  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
p>Features and Benefits:

  • No-wash steps, no separation steps
  • Compatible with high biotin samples, including RPMI and high-biotin tissue extracts
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Results in less than 3 hours

AlphaLISA technology allows the detection of molecules of interest in a no- wash, highly sensitive, quantitative assay. In an AlphaLISA competition assay, a Biotinylated analog of the analyte of interest, the tracer, binds to the Streptavidin-coated Alpha Donor beads, while the Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of low analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm (Figure 2). In the presence of high analyte, the beads are separated resulting in lower emission.

Progesterone is a steroid hormone that plays a role in the menstrual cycle and pregnancy. Progesterone directs pregnancy in a multitude of ways through changes in carbohydrate, protein, and lipid metabolism. Most progesterone is produced in the corpus luteum. This hormone plays an important role in the nervous system as a neurosteroid, where it serves as a precursor to allopregnanolone. Progesterone serves as a metabolic intermediate for many of the corticosteroids and sex hormones.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target Progesterone
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 5000 assay points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Metabolic
Unit Size 5,000 assay points

Resource Library & Events

Resource Type File Name File Format
Data Sheet Manual - AlphaLISA Progesterone AL335 PDF 803 KB