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Plasminogen (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Plasminogen (PLG) Detection Kit is designed for detection and quantitation of human plasminogen in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. This kit was designed to detect both plasminogen and plasmin.

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Product Number List Price Your Price Quantity
AL248C 1433.00 USD

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Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Plasminogen (PLG) is the 100 kDa inactive precursor of plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Synthesized in the kidney, PLG is found in plasma and many extracellular fluids. PLG deficiency is usually associated with either a thrombotic or a hyperfibrinolytic condition. Plasmin’s primary target is fibrin, but it is also able to degrade several constituents of the extracellular matrix and to convert a number of pro-hormones and cytokine precursors to their active form. Plasmin also appears to be involved in the metastatic spread of cancer.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Plasminogen
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Cardiovascular
Unit Size 500 assay points