The AlphaLISA® PER.C6® HCP Detection Kit is designed for the quantitative detection of PER.C6® contaminants in buffer and cell culture media using a homogeneous (no wash steps, no separation steps) assay.
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The PER.C6® human cell line is utilized as an expression system for the industrial scale production of human recombinant proteins or biotherapeutics. The PER.C6® cell line has several advantages over other platforms, such as increased yield and improved safety of the final product. Biotherapeutics for human use must be extensively purified to remove undesired contaminants, such as host cell proteins, that could elicit toxic or immunological reactions. Therefore, there is a current need to quantitate and trace these unwanted host cell proteins in protein preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target||PER.C6 HCP|
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|One Unit Contains||1 kit|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Application Note||A Comparison of AlphaLISA and TR-FRET Homogeneous Immunoassays in Serum-Containing Samples||PDF 1 MB|
|Technical Note||HiBlock Buffer Technical Data Sheet||PDF 333 KB|
|Data Sheet||Manual AlphaLISA PER.C6® HCP AL302||PDF 215 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|
|Poster||Efficient AlphaLISA immunoassays for the detection and characterization of monoclonal antibody biotherapeutics||PDF 525 KB|
|Poster||New AlphaLISA assays for the development of monoclonal antibody biotherapeutics||PDF 501 KB|
|Flyer||AlphaLISA - Frequently Asked Questions||PDF 102 KB|