PerkinElmer
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PDGF-BB/PDGFRβ (human) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRβ, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRβ.

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Part Number Unit Size List Price Your Price Quantity
AL3065C 500 Assay Points 1747.00 USD
AL3065F 5,000 Assay Points 14000.00 USD
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Overview

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

AlphaLISA technology allows detecting the binding of target proteins in a highly sensitive, quantitative, reproducible and user-friendly mode. In this AlphaLISA assay, a biotinylated PDGF-BB binds to the Streptavidin-coated Alpha Donor beads, while His tagged PDGFRβ is captured by Anti-His AlphaLISA Acceptor beads. When PDGF-BB binding to PDGFRβ happens, Donor beads and Acceptor beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Platelet-derived growth factor (PDGF) is one of numerous growth factors that regulate cell growth and division. PDGF is a dimeric glycoprotein that can be composed of two A subunits (PDGF-AA), two B subunits (PDGF-BB), or one of each (PDGF-AB). The dimeric isoforms are differentially expressed in various cell types and their effects are mediated through two cell surface tyrosine kinase receptors, platelet-derived growth factor receptors, termed alpha (PDGFRβ) and beta (PDGFRβ). Differences exist in isoform binding to each receptor. PDGF and PDGFR are involved in human cancer development and progression through autocrine stimulation of tumor cell growth. Blocking PDGF and PDGFR binding has been considered as a potential target for antitumor and antiangiogenic therapy.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target PDGF-BB, PDGFR
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 5,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Utilizing AlphaLISA Technology to Screen for Inhibitors of the CTLA-4 Immune Checkpoint PDF  1 MB
Manual Manual: AlphaLISA PDGF-BB/PDGFRb Binding Kit AL3065 PDF  664 KB