PerkinElmer
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PDGF (BB dimer) (human) AlphaLISA Detection Kit, 100 assay points

The AlphaLISA® immunoassay kit for human PDGF (BB homodimer) enables the quantitative determination of human platelet-derived growth factor (PDGF) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AL3049HV
100 Assay Points
600.00 USD
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AL3049C
500 Assay Points
1800.00 USD
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AL3049F
5,000 Assay Points
14400.00 USD
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Platelet-derived Growth Factor (PDGF) helps to regulate cell growth and division, specifically angiogenesis. PDGF is a dimeric glycoprotein. It can exist as a homodimer of the A chains, B chains, or as a heterodimer (AB). This kit detects the PDGF-BB homodimer. Mostly synthesized in platelets, PDGF can also be derived from smooth muscle cells, activated macrophages, and endothelial cells. Recombinant PDGF has been used to help heal chronic ulcers and to treat bone loss. The deregulation of PDGF signaling has been linked to many inflammatory diseases including: atherosclerosis, pulmonary hypertension and fibrosis.

Specifications

Assay Target PDGF
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 100 Assay Points