AlphaLISA® no-wash immunoassay kit for detection of human p62 (SQSTM1, Sequestosome 1) in buffered solution or cell lysates. The analyte in this kit consists of recombinant p62 fused to glutathione S-transferase (GST).
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The ubiquitin binding protein p62 or Sequestosome 1 (SQSTM1) is expressed in most cell types. Synonyms are p62 lck ligand, zeta interacting protein (ZIP, rat), A170 (mouse), OSIL or PDB3. The human protein is 440 amino acids long. It contains an N-terminal protein binding domain (PB1) that interacts with different protein kinases and allows homopolymerization of p62. The domain is followed by several other protein-protein interaction domains and nuclear localization and export signals. Notably, the C-terminal ubiquitin association domain recognizes both mono and polyubiquitin and the LIR motif allows direct binding to the autophagosome-associated protein LC3. p62 plays an important role in the formation of intracellular protein aggregates, autophagy, and NF-?B signaling. Mutation of p62 is associated with Paget’s disease (abnormal bone turnover).
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Application Note||A Comparison of AlphaLISA and TR-FRET Homogeneous Immunoassays in Serum-Containing Samples||PDF 1 MB|
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