PerkinElmer
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p24 (human HIV) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Immunodeficiency Virus type-1 p24 protein (HIV p24) Detection Kit is designed for detection and quantitation of HIV p24 in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
AL207C 500 assay points 1476.00 USD
AL207F 5,000 assay points 9800.00 USD
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Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

HIV p24 is the 230 aa phosphorylated protein of the Human Immunodeficiency Virus type 1 capsid forming the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. p24 is a cleavage product of the Gag polyprotein (between aa 132-133 and aa 363-364) by viral proteases. p24 is frequently used for HIV detection in blood, serum samples and other bodily fluids in acute HIV seroconversion, in neonatal infection and for monitoring of responses to antiviral drug therapy.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target p24
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Virology
Unit Size 500 assay points

Resources, Events & More

All (7)
Resource Type File Name File Format
Brochure Alpha Technology Solutions PDF  4 MB
Brochure Alpha product listing PDF  127 KB
Data Sheet Manual AlphaLISA HIV p24 AL207 PDF  194 KB
Flyer AlphaLISA - Frequently Asked Questions PDF  102 KB
Poster (All) All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures PDF  288 KB
Poster (All) Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines PDF  279 KB
Event Society of Laboratory Automation & Screening (SLAS) Event