The AlphaLISA® Human Immunodeficiency Virus type-1 p24 protein (HIV p24) Detection Kit is designed for detection and quantitation of HIV p24 in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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HIV p24 is the 230 aa phosphorylated protein of the Human Immunodeficiency Virus type 1 capsid forming the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. p24 is a cleavage product of the Gag polyprotein (between aa 132-133 and aa 363-364) by viral proteases. p24 is frequently used for HIV detection in blood, serum samples and other bodily fluids in acute HIV seroconversion, in neonatal infection and for monitoring of responses to antiviral drug therapy.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Data Sheet||Manual AlphaLISA HIV p24 AL207||PDF 194 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|
|Flyer||AlphaLISA - Frequently Asked Questions||PDF 102 KB|