The AlphaLISA® immunoassay kit for human OX40L enables the quantitative determination of human OX40 ligand in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
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For research use only. Not for use in diagnostic procedures.
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
OX40L Ligand (OX40L), also known as CD134L or TNFSF4, is a type II homotrimer transmembrane protein expressed on activated T and B cells as well as dendritic and endothelial cells. Binding to OX40 induces B7, CD40L, CD30L, CD27L, and 4-1BBL expression. Its major function is to transmit late co-stimulatory signals, which promote survival and proliferation of activated CD4+ T-cells which prolongs immune response. Other OX40 agonists are being evaluated for use as a potential cancer treatment.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 Assay Points|
Protocol for AlphaLISA human OX40L Detection Kit