PerkinElmer
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NNMT (human) AlphaLISA Detection Kit, 100 assay points

The AlphaLISA® immunoassay kit for human Nicotinamide N-Methyltransferase (NNMT) enables the quantitative determination of human NNMT in buffered solution, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL3079HV 100 Assay Points 728.00 USD
AL3079C 500 Assay Points 1747.00 USD
AL3079F 5,000 Assay Points 14000.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Nicotinamide N-methyltransferase (NNMT) is a cytosolic enzyme that is typically found in livers, however can still be expressed in other organs. This gene encodes the protein accountable for the enzymatic activity that uses S-adenosyl methionine as the methyl donor. NNMT is known to be a key enzyme in drug metabolism and is related to diseases such as hepatocellular carcinoma (HCC) and Parkinson’s disease. NNMT mRNA levels in patients with HCC are reduced whereas patients’ brains with Parkinson’s disease show high levels of this enzyme.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target NNMT
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 100 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual Protocol for AlphaLISA human NNMT detection assay PDF  455 KB