The AlphaLISA Mouse TNFα Detection Kit is designed for the quantitative determination of mouse TNFα in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
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In the mouse, Tumor Necrosis Factor alpha (TNFa) is primarily produced as a homotrimeric 235 amino acid membrane- bound protein. The soluble mature homotrimeric form of 156 amino acids is then released by the metalloprotease TNFa converting enzyme. In humans, TNFa is produced by many cell types like macrophages, monocytes, neutrophils, T cells, and NK cells. It causes cytolysis and cytostasis of many tumor cell lines in vitro. Within hours of injection, TNFa leads to the destruction of small blood vessels within malignant tumors. Although TNFa inhibits the growth of endothelial cells in vitro, it is a potent promoter of angiogenesis in vivo. In contrast to chemotherapeutic drugs, TNFa specifically attacks malignant cells. Furthermore, TNFa is associated with autoimmune disorders, and antibodies directed against TNFa have proven useful.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|One Unit Contains||5000 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Application Note||A Comparison of AlphaLISA and TR-FRET Homogeneous Immunoassays in Serum-Containing Samples||PDF 1 MB|
|Application Note||AlphaLISA Automation - Use of the JANUS Automated Workstation to automate AlphaLISA assays||PDF 870 KB|
|Application Note||Evaluating a TNF-alpha immunoassay using EnSpire AlphaPLUS ELISA and AlphaLISA technologies||PDF 185 KB|
|Data Sheet||Manual AlphaLISA Mouse TNFa AL505||PDF 261 KB|
|Poster||A Comparison of AlphaLISABead-Based Luminescence and Electrochemiluminescence Immunoassay Technologies for Detection of Human EPO, Amyloid Beta 42 and VEGF in Complex Sample Matrices||PDF 179 KB|
|Poster||A Comparison of a Homogeneous Bead-Based Amplified Luminescence Technology AlphaLISA and a Colorimetric ELISA for Detection of Human Matrix Metalloproteinase 9 in Complex Sample Matrices||PDF 1 MB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|