AlphaLISA® no-wash immunoassay kit for detection of mouse VEGF-A in buffer, cell culture supernatants, BALF (bronchoalveolar lavage fluid), lung homogenates, and other sample types. The analyte in this kit consists of the mouse VEGFA164.
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Mouse Vascular Endothelial Growth Factor (VEGF-A or VEGF) is a homodimeric 46 kDa heparin-binding glycoprotein specific for endothelial cells. Mouse VEGF is the product of a single gene containing eight exons, which produces three splice variants containing 120, 164, and 188 amino acid residues. The various isoforms have different heparin binding properties as well as solubility characteristics reflecting the different functional properties of the VEGF forms. VEGF is believed to play important roles in inflammation and during normal and pathological angiogenesis, a process that is associated with wound healing, embryonic development, and growth and metastasis of solid tumors. Elevated levels of VEGF have been observed in synovial fluids of rheumatoid arthritis patients and sera of cancer patients.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Technical Note||HiBlock Buffer Technical Data Sheet||PDF 333 KB|
|Data Sheet||Manual AlphaLISA Mouse VEGF-A AL520||PDF 234 KB|
|Poster||A Comparison of AlphaLISABead-Based Luminescence and Electrochemiluminescence Immunoassay Technologies for Detection of Human EPO, Amyloid Beta 42 and VEGF in Complex Sample Matrices||PDF 179 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|