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IL-2 (mouse) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Mouse Interleukin-2 (IL-2) Detection Kit is designed for detection and quantitation of mouse IL-2 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number Unit Size List Price Your Price Quantity
AL511C 500 assay points 1433.00 USD
AL511F 5,000 assay points 9500.00 USD

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Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

In the mouse, Interleukin 2 (IL2), formerly known as T-cell growth factor (TCGF), contains 149 amino acids in its mature form. In humans, this immunoregulatory lymphokine is produced by lectin- or antigen-activated T cells and capable of inducing the thymic expansion of recently activated antigen-specific T lymphocytes. However, since T-cell immunity could be elicited by various agents in the absence of IL2 in vivo, it is currently thought that the main non-redundant activity of this cytokine is the induction of the suppressor function of CD4+CD25+ regulatory T cells (Treg) in peripheral lymph nodes to ensure suppression of autoreactive T cells that escape negative selection. Moreover, recent findings suggest that IL2-mediated regulation of Treg cells is important in the prevention of type 1 diabetes and autoimmune disease.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL2
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Data Sheet Manual AlphaLISA Mouse IL-2 AL511 PDF 209 KB
Poster AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices PDF 273 KB