The AlphaLISA® Mouse Interleukin-15 (IL-15) Detection Kit is designed for detection and quantitation of mouse IL-15 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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In the mouse, Interleukin 15 (IL15) is a cytokine produced as a precursor maturing into a 114 amino acid glycoprotein. In human, IL15 is generated by multiple tissues (placenta, skeletal muscle, kidney, lung, heart, and monocytes/macrophages). IL15 acts mainly on T and B cells and it is well known as a key cytokine in natural killer cell development, proliferation, and survival. IL15 binds with high affinity to the IL15 receptor a and forms a complex with the a and ? chains of the IL2 receptor for signal transduction. It can regulate inflammatory response and NK cell activation during immune responses. IL15 signalling has become a focus of interest for therapeutic intervention especially in autoimmune diseases like inflammatory bowel disease, systemis lupus erythematosus, inflammatory synivitis, psoriasis, diabetes mellitus, asthma bronchiale, rheumatoid arthritis, and T-cell leukemia.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|One Unit Contains||500 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Mouse IL-15 AL513||PDF 212 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|