The AlphaLISA® Human Matrix Metalloproteinase-2 (MMP2) Detection Kit is designed for detection and quantitation of human MMP2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP2 and pro-MMP2.
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For research use only. Not for use in diagnostic procedures.
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Matrix Metalloproteinase 2 (MMP2) or type IV collagenase contains 551 amino acids in its mature state. MMP2 is mainly expressed in placenta, lung, pancreas, ovary, spleen, and intestine. MMP2 is secreted as a pro-enzyme (pro-MMP2) and is activated by proteolytic cleavage by other MMPs. The family of tissue inhibitor metalloproteinases (TIMPs) controls the balance between pro and active forms of MMPs. The association of MMP2 with integrin alpha-v-beta3 is important for mesenchymal cell invasion and angiogenesis. MMP2 is over-expressed in inflammatory bowel diseases. Mutations in this gene have been associated with Torg-Winchester syndrome, also called Multicentric Osteolysis Nodulosis and Arthropathy (MONA).
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.