The AlphaLISA® Mouse Interleukin-6 (IL-6) Detection Kit is designed for detection and quantitation of mouse IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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In the mouse, Interleukin 6 (IL6) is a 187 amino acid cytokine. In humans, IL6 is produced by a variety of cell types, including T-cells, mast cells, and monocytes. IL6 signals through binding to the gp130/ IL6R receptor complex. IL6 acts not only on the immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory responses, depending on the target cell analyzed and the in vivo environmental circumstances. IL6 is a differentiation and proliferation factor for B and T cells, and acts as a migration factor on monocytic cells. It is the major activator of acute-phase protein expression in the liver and acts as a survival factor on neuronal cells. Elevated serum IL6 levels have been observed during bacterial and viral infection, trauma, autoimmune disease, inflammation, and cancer.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|One Unit Contains||5000 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Mouse IL-6 AL504||PDF 228 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|