The AlphaLISA® Mouse Interleukin-1ß (IL-1ß) Detection Kit is designed for detection and quantitation of mouse IL-1ß in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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The mouse Interleukin 1 beta (IL1ß) is produced as a 269 amino acid precursor that matures by proteolysis to its 152 amino acid active form. In humans, IL1a and IL1ß are central players of the immune response, displaying roles in inflammation both at local and systemic levels. IL1ß is functionally equivalent to IL1a. Its production has been reported in many cell types including brain cells, as well as monocytic and peripheral blood mononuclear cells. Among the biological activities of IL1 is the stimulation of T-helper cells, which then secrete IL2 and express IL2 receptor. IL1 acts directly on B-cells, promoting their proliferation and the synthesis of immunoglobulins. It supports tumor cytotoxicity mediated by monocytes and induces tumor regression. It has been shown that IL1 also promotes wound healing.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.