check quantity

Leptin (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Leptin Detection Kit is designed for detection and quantitation of human leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Due to the presence of leptin binding proteins in serum or other matrices, an acid-dissociation step might be required before performing the AlphaLISA assay.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Product Number Unit Size List Price Your Price Quantity
AL225C 500 assay points 1433.00 USD
AL225F 5,000 assay points 9500.00 USD

You successfully added item(s) to your cart

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Leptin is a 16-kDa hormone secreted by differentiated adipocytes. It regulates energy homeostasis as a result of its action on the brain via hypothalamic neuronal pathways expressing the leptin receptor (LR). Hereditary deficiency of leptin, or functional LRs, causes severe obesity in humans and mice. Leptin-mediated signaling has been implicated in the regulation of food intake, energy expenditure, lipid and carbohydrate metabolism, and reproductive, neuroendocrine, thyroid, and immune function.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Leptin
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Metabolic
Unit Size 5,000 assay points