check quantity

IP-10 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Product Number Unit Size List Price Your Price Quantity
AL326HV 100 assay points 608.00 USD
AL326C 500 assay points 1433.00 USD
AL326F 5,000 assay points 9500.00 USD

You successfully added item(s) to your cart

Overview

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interferon ?-induced protein 10 (IP10) is also known as IC-X-C motif chemokine 10 (CXCL10) or small-inducible cytokine B10. It is an 8.7 kDa protein encoded by the CXCL10 gene and belongs to the CXC chemokine family. IP10 is secreted by several types of immune cells in response to IFN? stimulation. Increased levels appear to be a pre-treatment marker for interferon/ribavirin therapy in HCV and HIV infected patients. The present kit permits the detection of human IP10 (i.e. analyte) in human serum, plasma, and immune cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IP10
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 5,000 assay points