The AlphaLISA® Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
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Interferon ?-induced protein 10 (IP10) is also known as IC-X-C motif chemokine 10 (CXCL10) or small-inducible cytokine B10. It is an 8.7 kDa protein encoded by the CXCL10 gene and belongs to the CXC chemokine family. IP10 is secreted by several types of immune cells in response to IFN? stimulation. Increased levels appear to be a pre-treatment marker for interferon/ribavirin therapy in HCV and HIV infected patients. The present kit permits the detection of human IP10 (i.e. analyte) in human serum, plasma, and immune cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|One Unit Contains||1 kit|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 assay points|
|Resource Type||File Name||File Format|
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