The AlphaLISA® Human Interleukin-3 (IL3) Detection Kit is designed for detection and quantitation of human IL-3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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Interleukin 3 (IL3) is a hematopoietic colony-stimulating factor that induces the proliferation and differentiation of several hematopoietic/lymphoid cell types and also has neurotrophic activity. It is a ~28 kDa glycoprotein (133-140 amino acids long) produced by antigen-activated T cells, eosinophils and mast cells in tissues under allergic inflammation. IL3 has been given several designations such as mast-cell growth factor, burst-stimulating activity, and multi-colony-stimulating factor because of its potent growth-promoting activities. IL3 signals via the IL3R which shares a common signal transducing chain with IL5 and GM-CSF. However, their effects are not identical, suggesting they may activate substantially different pathways. It has been shown to enhance haematopoiesis in different animal models, and to accelerate platelet and neutrophil recovery in humans after myelotoxic chemotherapy or bone marrow transplantation.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|One Unit Contains||500 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Technical Note||HiBlock Buffer Technical Data Sheet||PDF 333 KB|
|Data Sheet||Manual AlphaLISA Human IL3 AL222||PDF 217 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|