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IL-3 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Interleukin-3 (IL3) Detection Kit is designed for detection and quantitation of human IL-3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number List Price Your Price Quantity
AL222C 1433.00 USD

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  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin 3 (IL3) is a hematopoietic colony-stimulating factor that induces the proliferation and differentiation of several hematopoietic/lymphoid cell types and also has neurotrophic activity. It is a ~28 kDa glycoprotein (133-140 amino acids long) produced by antigen-activated T cells, eosinophils and mast cells in tissues under allergic inflammation. IL3 has been given several designations such as mast-cell growth factor, burst-stimulating activity, and multi-colony-stimulating factor because of its potent growth-promoting activities. IL3 signals via the IL3R which shares a common signal transducing chain with IL5 and GM-CSF. However, their effects are not identical, suggesting they may activate substantially different pathways. It has been shown to enhance haematopoiesis in different animal models, and to accelerate platelet and neutrophil recovery in humans after myelotoxic chemotherapy or bone marrow transplantation.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target IL3
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 500 assay points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 500 assay points