The AlphaLISA® immunoassay kit for bovine IL-2 enables the detection and quantitation of bovine interleukin-2 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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For research use only. Not for use in diagnostic procedures.
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Cytokines are soluble mediators that impact a multitude of biologies including cell proliferation, survival, death, motility, cell-cell and cell-matrix interactions as well as immune response and leukocyte infiltration. Cytokines are intimately associated with acute and chronic diseases and response to vaccination. IL2 plays key roles in tolerance and immunity, primarily via its direct effects on T cells. Bovine Interleukin 2 (bIL2) is a 15 kDa cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. It plays a key role in promoting clonal expansion of antigen-specific T cells. It can stimulate the growth and expansion of a multitude of cells as well as induce cytokine production and cytolytic activity by natural killer cells.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|