The AlphaLISA® Human Interleukin 1 alpha (IL1α) Detection Kit is designed for detection and quantitation of human IL1α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL238C||500 assay points||1476.00 USD|
|AL238F||5,000 assay points||9800.00 USD|
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Interleukin 1a (IL1a) is an 18 kDa pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. The IL1 family includes the agonists IL1a and IL1ß and the antagonist IL1RA. These three molecules compete for the binding to type 1 IL1 receptor (IL-1R1). IL1a is produced as a pro-protein by macrophages and monocytes, and is processed to its active form as a response to cell injury, inducing cell apoptosis. IL1a gene polymorphism has been associated with Alzheimer’s disease and rheumatoid arthritis.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Technical Note||HiBlock Buffer Technical Data Sheet||PDF 333 KB|
|Data Sheet||Manual AlphaLISA Human IL1a AL238||PDF 267 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|