The AlphaLISA® Human Interleukin 18 (IL-18) Detection Kit is designed for detection and quantitation of human IL-18 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL241C||500 assay points||1476.00 USD|
|AL241F||5,000 assay points||9800.00 USD|
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Interleukin 18 (IL18) is a 18 kDa cytokine produced by Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, and adrenal cortex cells. IL18 is involved in the production of interferon-? (IFN-?) in response to toxic shock and shares functional similarities with IL12. IL18 works together with IL12 to induce cell-mediated immunity following infection with microbial products like lipopolysaccharides (LPS). IL18 is also able to induce severe inflammatory reactions, which suggests a role in certain inflammatory disorders. Elevated plasma levels of IL18 have been reported in hematological malignancies, inflammatory diseases, and autoimmune disorders.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Brochure||Alpha product listing||PDF 132 KB|
|Data Sheet||Manual AlphaLISA Human IL18 AL241||PDF 212 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|