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IL-18 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Interleukin 18 (IL-18) Detection Kit is designed for detection and quantitation of human IL-18 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number List Price Your Price Quantity
AL241C 1433.00 USD

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  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin 18 (IL18) is a 18 kDa cytokine produced by Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, and adrenal cortex cells. IL18 is involved in the production of interferon-? (IFN-?) in response to toxic shock and shares functional similarities with IL12. IL18 works together with IL12 to induce cell-mediated immunity following infection with microbial products like lipopolysaccharides (LPS). IL18 is also able to induce severe inflammatory reactions, which suggests a role in certain inflammatory disorders. Elevated plasma levels of IL18 have been reported in hematological malignancies, inflammatory diseases, and autoimmune disorders.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target IL18
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 500 assay points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 500 assay points