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IL-15 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Interleukin 15 (IL15) Detection Kit is designed for detection and quantitation of human IL-15 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number List Price Your Price Quantity
AL283F 9500.00 USD

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Details

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Human Interleukin 15 (IL15) is a cytokine produced as a precursor maturing into a 114 amino acid glycoprotein. IL15 is generated by multiple tissues (placenta, skeletal muscle, kidney, lung, heart, and monocytes/macrophages). IL15 acts mainly on T and B cells and it is well known as a key cytokine in natural killer cell development, proliferation, and survival. IL15 binds with high affinity to the IL15 receptor a and forms a complex with the a and ? chains of the IL2 receptor for signal transduction. It can regulate inflammatory response and NK cell activation during immune responses. IL15 signalling has become a focus of interest for therapeutic intervention especially in autoimmune diseases like inflammatory bowel disease, systemis lupus erythematosus, inflammatory synivitis, psoriasis, diabetes mellitus, asthma bronchiale, rheumatoid arthritis, and T-cell leukemia.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target IL15
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 5000 assay points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 5,000 assay points