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Il-12 (p70) (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Interleukin 12 (p70) Detection Kit is designed for detection and quantitation of human IL-12 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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Product Number Unit Size List Price Your Price Quantity
AL239C 500 assay points 1433.00 USD
AL239F 5,000 assay points 9500.00 USD

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  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin 12 (p70) (IL12 (p70)) is a disulfide-linked heterodimeric cytokine formed of 2 subunits, one of 35 kDa and the other of 40 kDa, and is produced mainly by monocytes, macrophages, B-lymphocytes, and dendritic cells. This cytokine plays a central role in promoting the differentiation of naive CD4+ T cells into mature TH1 effector cells and is a potent stimulus for NK cells and CD8+ T cells to produce IFN-?. IL12 appears to play a major role in auto-immune disease, in the resistance to bacterial and parasitic infections, in antiviral responses, and in the promotion of antitumor immunity. Increased plasma levels of IL12 have been reported in certain neurological disorders and auto-immune diseases.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target IL12
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 5000 assay points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 5,000 assay points