The AlphaLISA® Human Interleukin 11 (IL-11) Detection Kit is designed for detection and quantitation of IL-11 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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Human interleukin 11 (IL11) is a non-glycosylated protein of 23 kDa (179 amino acids). It is produced by bone marrow stromal cells (fibroblasts) and also by a variety of mesenchymal cells. IL11 stimulates megakaryocytopoiesis, activates osteoclasts, inhibits epithelial cell proliferation and apoptosis, and inhibits macrophage mediator production. IL-11 also possesses anti-inflammatory activity, and has been proposed as a therapeutic agent in the treatment of chronic inflammatory diseases, such as Crohn's disease and rheumatoid arthritis. IL11 activity is mediated by the IL-11 receptor (IL-11R) that has been reported in a wide variety of cells and tissues. IL11 binds to the IL-11R protein alone with low affinity; the affinity increases when IL-11R is associated with is signal transducer: gp130.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|One Unit Contains||500 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Technical Note||HiBlock Buffer Technical Data Sheet||PDF 333 KB|
|Data Sheet||Manual AlphaLISA Human IL-11 AL293||PDF 229 KB|
|Poster||A Comparison of AlphaLISABead-Based Luminescence and Electrochemiluminescence Immunoassay Technologies for Detection of Human EPO, Amyloid Beta 42 and VEGF in Complex Sample Matrices||PDF 179 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|