PerkinElmer
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IL-21 (human) AlphaLISA Detection Kit, 500 assay points

The AlphaLISA® immunoassay kit for human IL-21 enables the quantitative determination of human interleukin-12 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL3043HV 100 Assay Points 582.00 USD
AL3043C 500 Assay Points 1747.00 USD
AL3043F 5,000 Assay Points 14000.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Interleukin 21 (IL21) is a 15 kDa cytokine that is produced by activated T follicular helper cells and natural killer T cells. It has regulatory effects on cells of the immune system such as cytoxic T cells, which destroy infected or damaged cancerous cells. In addition, IL21 is known to suppress cutaneous hypersensitivity reactions by limiting allergen specific IgE production and mast cell degranulation. High levels of IL21 are related to decreasing allergic responses, as well as controlling persistent viral infections, metastatic melanoma, and renal cell carcinoma. This kit is designated to detect and quantify the levels of IL21 in cell culture media and other biological matrices.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL21
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Application Note Utilization of AlphaLISA Technology to Accurately Detect Asthma Biomarkers in Human Serum PDF  1 MB
Manual AlphaLISA Human IL-21 Detection kit AL3043 PDF  491 KB