The AlphaLISA® Human Insulin-like Growth Factor 1 (IGF1) Detection Kit is designed for detection and quantitation of human IGF1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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Insulin-like Growth Factor 1 (IGF1 or Somatomedin C) is 70 amino acids in length after being fully processed. IGF1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. IGF1, via binding to the IGF receptor, acts as natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication as well as a potent inhibitor of programmed cell death. IGF1 mediates many of the growth-promoting effects of growth hormone by promoting the incorporation of sulfate into cartilage in many normal tissues. The insulin-like growth factor pathway plays a major role in cancer cell proliferation, survival and resistance to anti-cancer therapies underlying the importance of IGF1 as a serum biomarker in various diseases such as diabetes and cancer.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|One Unit Contains||5000 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Data Sheet||Manual AlphaLISA Human IGF1 AL236||PDF 214 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|