The AlphaLISA® Human IgA Protein Detection Kit is designed for detection and quantitation of human IgA in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
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Immunoglobulin A (IgA) is a class of antibody mainly present in mucosal areas. IgA is essentially secreted in a dimeric form, which is maintained in place by the J-chain, a polypeptide of 15 kDa rich in cysteine residues. There are two isotypes of IgA: IgA1 is prevalent in the serum while IgA2 is prevalent in secretions. IgA is present in the gut, respiratory tract, urogenital tract, and in mucous secretions like saliva, tears, colostrum, vaginal fluid, and breast milk. IgA is relatively resistant to degradation and can remain active in extreme environments such as the digestive tract. IgA binds to pathogen, but is a poor activator of the complement. IgA deposition in the glomerulus is associated with IgA nephropathy.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Antibody|
|Experimental Type||In vitro|
|One Unit Contains||5000 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Human IgA AL262||PDF 203 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|