PerkinElmer
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IFN-γ (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA Human IFN-γ Detection Kit is designed for the quantitative determination of human IFN-γ in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
AL217C 500 assay points 1476.00 USD
AL217F 5,000 assay points 9800.00 USD
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Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interferons (IFNs) activity has been discovered due to their antiviral effects. In humans, there are three families of IFNs: IFN type I (IFN-a, ß, ?, e, and ?), IFN type II (one single representative, IFN-?), and IFN type III (IFN-?1-3). Antigens and mitogens stimulate in Natural Killer (NK) and activated helper T lymphocytes (Th1) the production of IFN-?. Human IFN-? is a 140 amino acids polypeptide that shows multiple effects; it induces the production of cytokines, upregulates the expression of class I and II MHC antigens, and leukocyte adhesion molecules. It also activates macrophages, enhances the secretion of immunoglobulins by B cells, and potentiates Th1 cell expansion. Response to IFN-? is mediated by the hetetodimeric IFN-? Receptor, triggering a signalling cascade involving JAK1, JAK2, and STAT1. Importantly, IFNs have been proved to be effective in the treatment of several viral infections and cancers.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IFNγ
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 5,000 assay points

Resources, Events & More

All (12)
Resource Type File Name File Format
Brochure Alpha product listing PDF  127 KB
Application Note A Comparison of AlphaLISA and TR-FRET Homogeneous Immunoassays in Serum-Containing Samples PDF  1 MB
Application Note AlphaLISA Automation - Use of the JANUS Automated Workstation to automate AlphaLISA assays PDF  870 KB
Application Note Evaluating a TNF-alpha immunoassay using EnSpire AlphaPLUS ELISA and AlphaLISA technologies PDF  185 KB
Technical Note HiBlock Buffer Technical Data Sheet PDF  333 KB
Data Sheet Manual AlphaLISA Human IFN? AL217 PDF  288 KB
Poster (All) A Comparison of AlphaLISABead-Based Luminescence and Electrochemiluminescence Immunoassay Technologies for Detection of Human EPO, Amyloid Beta 42 and VEGF in Complex Sample Matrices PDF  179 KB
Poster (All) A Comparison of a Homogeneous Bead-Based Amplified Luminescence Technology AlphaLISA and a Colorimetric ELISA for Detection of Human Matrix Metalloproteinase 9 in Complex Sample Matrices PDF  1 MB
Poster (All) All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures PDF  288 KB
Poster (All) AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices PDF  273 KB
Poster (All) Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines PDF  279 KB
Event Society of Laboratory Automation & Screening (SLAS) Event