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ICAM-1 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Intercellular Adhesion Molecule-1 (ICAM-1) Detection Kit is designed for detection and quantitation of human ICAM-1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies target the extracellular domain of ICAM-1. The analyte in this kit consists of the extracellular domain of human ICAM-1.

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Product Number Unit Size List Price Your Price Quantity
AL282C 500 assay points 2745.00 USD
AL282F 5,000 assay points 21500.00 USD

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  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Intercellular Adhesion Molecule-1 (ICAM-1) is a dimeric transmembrane glycoprotein with a molecular weight of 80 - 114 kDa. ICAM-1 is a member of the immunoglobulin family and can be expressed on non-hematopoietic cells, such as vascular endothelial cells. It is involved in the transmigration of leucocytes to sites of inflammation. A soluble form of ICAM-1 (sICAM-1) has been identified in serum and cerebrospinal fluid and it contains the five extracellular Ig-domains of ICAM-1. sICAM-1 is an inflammatory marker that has been associated with several common diseases such as diabetes, heart disease, stroke, and malaria. High levels of sICAM-1 have been found in serum from patients with cardiovascular disease, cancer, HIV-1, and autoimmune diseases. sICAM-1 is able to competitively bind the ligands of membrane-bound ICAM-1, such as LFA-1, Mac-1, and human rhinovirus.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target ICAM-1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 500 assay points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Cardiovascular
Unit Size 500 assay points

Resource Library & Events

Resource Type File Name File Format
Data Sheet Manual AlphaLISA human ICAM-1 AL282 PDF 228 KB
Poster AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices PDF 273 KB