PerkinElmer
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TDP-43 (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for TDP-43 is designed for the quantitative determination of Transactive response DNA binding protein 43 (TDP43) in human serum, CSF, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL387HV 100 assay points 604.00 USD
AL387C 500 assay points 2266.00 USD
AL387F 5,000 assay points 19600.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

TAR DNA-binding protein 43 (TDP-43, transactive response DNA binding protein 43 kDa) is a transcriptional repressor protein. Excess amounts of protein inside the nucleus of nerve cells have been reported in two fatal neurodegenerative diseases - amyotrophic lateral sclerosis (ALS), and fronto-temporal dementia (FTD). As such, it is becoming an important biomarker of neurological disease and target for intervention. This AlphaLISA kit has been designed to detect and quantify Human TDP-43 in human serum, CSF, cell culture supernatants, and cell lysates.p>

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target TDP-43
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Central Nervous System
Unit Size 100 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Data Sheet AlphaLISA TDP-43 (Human) Detection Kit PDF  345 KB
Event Society of Laboratory Automation & Screening (SLAS) Event