PerkinElmer
check quantity

Oncostatin M (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Oncostatin M Detection Kit is designed for detection and quantitation of oncostatin M in cell culture media, buffer solution, and serum using a homogeneous (no-wash steps, no separation steps) assay.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Part Number Unit Size List Price Your Price Quantity
AL341HV 100 assay points 675.00 USD
AL341C 500 assay points 1476.00 USD
AL341F 5,000 assay points 9800.00 USD
Buy Now

You successfully added item(s) to your cart

Overview

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Oncostatin M, also known as OSM, is a growth factor encoded by the OSM gene in humans. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines (also include granulocyte colony-stimulating factor), most closely resembles leukemia inhibitory factor (LIF) in both structure and function. The fully active 196 residue OSM is the predominant form isolated from activated monocytes and T lymphocytes, and corresponds to a glycoprotein of 28 Kda. It is proving important in liver development, haematopoeisis, inflammation and possibly CNS development. It is also associated with bone formation and destruction. It is a growth regulator which inhibits the proliferation of a number of tumor cell lines and stimulates normal fibroblasts, AIDS-Kaposi’s sarcoma. It regulates cytokine production, including IL-6, G-CSF (granulocyte colony-stimulating factor) and GM-CSF from endothelial cells. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Oncostatin M
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Data Sheet Manual: AlphaLISA human oncostatin M Detection Kit AL341 PDF  456 KB
Event Society of Laboratory Automation & Screening (SLAS)