PerkinElmer
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N-cadherin (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human N-cadherin enables the detection and quantitation of human N-Cadherin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL379HV 100 assay points 445.00 USD
AL379C 500 assay points 1475.00 USD
AL379F 5,000 assay points 9700.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

N-Cadherin (NCAD or Cadherin-2) is a 99.7 kDa (906 AA) transmembrane protein composed of 5 extracellular cadherin repeats. It is expressed in a variety of tissues including cardiac and neural tissues, where its role is to mediate cell-cell interactions by interacting with cadherins on adjacent cells. N-Cadherin is commonly found at elevated levels in some cancer cells and due to its role in epithelial –mesenchymal transition it is thought to play a vital role in cancer metastasis. As such, N-cadherin has garnered attention as an important biomarker. This AlphaLISA kit has been designed to detect and quantify N-Cadherin in cell lysate and tissue homogenates.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target N-cadherin
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 100 assay points