AlphaLISA no-wash immunoassay kit for detection and quantitation of human MMP13 (matrix metalloproteinase-13, or Collagenase 3) in serum, plasma, cell culture supernatants, and other samples.
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|Product Number||Unit Size||List Price||Your Price||Quantity|
|AL318HV||100 assay points||608.00 USD|
|AL318C||500 assay points||1433.00 USD|
|AL318F||5,000 assay points||9500.00 USD|
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Matrix metalloproteinase 13 (MMP13, Collagenase 3), a member of the matrix metalloproteinase family, is an enzyme composed of a catalytic domain and a hemopexin-like domain, activated by cleavage of inactive pro-proteins by extracellular proteinases. MMP13 is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis of cancer.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Quantity in a Package Amount||1.0 per pack|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Human MMP13 AL318||PDF 233 KB|