PerkinElmer
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Hyaluronic acid (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human hyaluronic acid enables the detection and quantitation of Human Hyaluronic Acid (hHA) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human collagen Type I, Type II, Type III, and fibronectin.

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Part Number Unit Size List Price Your Price Quantity
AL354HV 100 assay points 675.00 USD
AL354C 500 assay points 1433.00 USD
AL354F 5,000 assay points 9500.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Hyaluronic Acid (HA), also known as hyaluronan or hyaluronate, is a large molecule (MV> 500KD) and is distributed throughout the body connective tissues. HA is an important component of synovial fluid, articular cartilage, skin, and extracellular matrix and play critical role in joints, cartilage, and skin health and the integrity of cells and cell functions. It has been extensively used in skin care products (cosmetic are) and in un-healthy joints treatments. It has been reported that the patients with fibrosis has significant increases in HA in serum and plasma. The present kit is designed to detect hHA in serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In this AlphaLISA assay, a biotinylated human aggrecan binds to the Streptavidin-coated Donor beads and human aggrecan is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Hyaluronic Acid
Assay Target Class Aminoglycan
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 100 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual AlphaLISA hHA Detection Kit AL354 PDF  386 KB
Event Society of Laboratory Automation & Screening (SLAS)