PerkinElmer
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COX-2 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human COX-2 enables the detection and quantitation of human COX-2 in cell culture supernatants and cell extracts using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human COX-1 nor with sheep COX-2.

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Part Number Unit Size List Price Your Price Quantity
AL376C 500 assay points 4101.00 USD
AL376F 5,000 assay points 30300.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

COX-2 is a homodimer of 70 kDa subunits that is also known as cyclooxygenase-2, prostaglandin epoxide synthase or prostaglandin G synthase. It is a heme-bearing membrane associated enzyme whose function is to attach an epoxy group to arachidonic acid, creating prostaglandins G or H. This epoxide is then used by other enzymes to generate prostaglandins with oxygen groups on their structure. COX-2 is one of the most important mediators of inflammation since the molecules it generates can activate cells to produce inflammatory cytokines. Also, neurons involved in pain detection bear receptors for these prostaglandins, making it one of the prime target for anti-pain analgesics including the popular aspirin (salycilyc acid), Tylenol™ (acetaminophen) or Advil™ and Motrin™ (ibuprophen).

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target COX-2
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Shipping Condition Dry Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Data Sheet AlphaLISA human COX-2 Detection Kit AL376 PDF  585 KB