PerkinElmer
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Claudin 7 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human claudin 7 enables the detection and quantitation of claudin 7 in cell culture media and human cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL381HV 100 assay points 676.00 USD
AL381C 500 assay points 2536.00 USD
AL381F 5,000 assay points 19000.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Claudin 7 belongs to a family of 24 tight junction (TJ) membrane proteins which are central for the regulation of cell permeability and the maintenance of cell polarity. Recent antibody-based studies indicated that Claudin 7 is not only localized at the apical TJs but also have a strong basolateral membrane distribution in the epithelia of various tissues. Abnormal expression and mislocalization of Claudin 7 are frequently observed in epithelial-derived lung, breast, head, and neck cancers. Measuring the Claudin 7 levels is important for the detection of different types of cancers and is also an important target for therapeutic intervention.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Claudin 7
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Data Sheet AlphaLISA human Claudin 7 Detection Kit AL381 PDF  578 KB
Poster Comparison of EMT biomarker expression in 2D monolayer and 3D spheroid cultures in a prostate cancer PDF  1 MB