The AlphaLISA® immunoDetection Kit for Hepatitis B Virus Surface antigen (HBsAg) enables the quantitative determination of HBsAg in cell culture media and buffer using a homogeneous AlphaLISA assay (no wash steps).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL3083HV||100 Assay Points||750.00 USD|
|AL3083C||500 Assay Points||1800.00 USD|
|AL3083F||5,000 Assay Points||14800.00 USD|
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Hepatitis B surface antigen (HBsAg) is a protein from Hepatitis B virus (HBV). HBV circulates in infected blood when the virus is actively replicating. Infection with HBV induces a spectrum of clinical manifestations and chronic liver diseases. HBsAg can be found on the surface of the lipid envelope containing HBeAg. These antigens are recognized by antibody proteins which bind them. Testing for surface antigens are simple way of detecting the presence of the Hepatitis B virus.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 128 KB|
|Manual||Protocol for AlphaLISA Heptatis B Virus (s Antigen) Kit||PDF 426 KB|