This kit is designed for the detection of di/mono-methylated Histone H3 Lysine 27 (H3K27me2-1) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
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The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput.
The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target||Histone H3|
|Assay Target Class||Histone|
|Experimental Type||In vitro|
|One Unit Contains||100 assay points|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||1 Kit|
|Resource Type||File Name||File Format|
|Brochure||Epigenetics Drug Discovery Solutions||PDF 1 MB|
|Technical Note||AlphaLISA Di/Mono-Methyl-Histone H3 Lysine 27 (H3K27me2-1) Cellular Detection Kit||PDF 903 KB|
|Poster||Characterization of Intractable Epigenetics Targets Using Homogenous Immunoassays||PDF 1 MB|
|Poster||Comparison of endogenous methyl marks on histone H3 in cancer-derived cell lines using AlphaLISA||PDF 1 MB|