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AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit, 100 Assay Points

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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Product Number Unit Size List Price Your Price Quantity
AL720HV 100 assay points 584.00 USD
AL720C 500 assay points 2035.00 USD
AL720F 5,000 assay points 11700.00 USD

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Overview

The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput.

  • Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns
  • Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models
  • Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6

The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Histone H3
Assay Target Class Histone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Acetylation
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Epigenetics
Unit Size 100 assay points

Resources, Events & More

Resource Type File Name File Format
Poster Comparison of endogenous methyl marks on histone H3 in cancer-derived cell lines using AlphaLISA PDF 1 MB