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GLP-1 (7-36 amide) (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human GLP-1 Detection Kit is designed for detection and quantitation of human GLP-1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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AL215C 1433.00 USD

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Details

One antibody recognizes the free N-terminus of GLP-1 (7-36 amide) (mouse monoclonal antibody). The second antibody recognizes the C-terminus of GLP-1 (7-36 amide) (mouse monoclonal antibody).

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

The Glucagon-Like Peptide-1 [GLP-1 (7-36 amide)] is a 30-31 oligopeptide, generated from proglucagon, secreted by the enteroendocrine L cells of the small and large intestine, in a nutrient-dependent manner (some GLP-1 (7-36 amide) is also produced by the pancreatic a-cells and in the central nervous system). Circulating GLP-1 (7-36 amide) levels rapidly increase shortly after ingestion, playing a significant role in the inhibition of gastric emptying and food intake. It is also important for blood glucose homeostasis through the stimulation of insulin biosynthesis and secretion, islet proliferation, and the inhibition of glucagon secretion. Moreover, it regulates hypothalamic-pituitary function and GLP-1 activated circuits mediate the central nervous system response to aversive stimulation. In the circulation, the active form of GLP-1 (GLP-1 (7-36 amide)) is promptly inactivated by the dipeptidyl peptidase IV (DP IV).

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target GLP-1
Assay Target Class Peptide
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 500 assay points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Metabolic
Unit Size 500 assay points