PerkinElmer
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GITR (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human GITR enables the quantitative determination of human glucocorticoid-induced TNFR-related protein (GITR) in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AL3101HV
100 Assay Points
750.00 USD
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AL3101C
500 Assay Points
2123.00 USD
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AL3101F
5,000 Assay Points
10600.00 USD
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Glucocorticoid-induced TNF-related Protein (GITR), also known as TNFSF18, is a transmembrane receptor protein expressed on peripheral blood T cells and in bone marrow. It shares homology with 4-1BB and CD27. Ligation of GITR activates NfκB and protects from TCR activated cell death. Activation of GITR by anti-GITR antibodies has been shown to be effective in inducing antitumor immune response.

Specifications

Assay Target GITR
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 5,000 Assay Points
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Manual

Protocol for AlphaLISA human GITR Detection Kit

Protocol for AlphaLISA human GITR Detection Kit

PDF 600 KB