The AlphaLISA® Human Growth Hormone Detection Kit is designed for detection and quantitation of human growth hormone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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Human Growth Hormone (GH, GHN or GH1) is the major regulator of postnatal growth, having somatogenic, metabolic, and differentiative effects on its target cells and tissues. It is a 22 kDa protein (191 amino acids) belonging to a family of cytokine peptides, and is produced and secreted by acidophilic or somatotropic cells of the anterior pituitary gland. GH binds to its cognate transmembrane receptor (GHR), and signals through the activation of JAKs, STATs, AKT, and ERK. It exerts many of its effects by stimulation of IGF-1 (Insulin-like Growth Factor I) production in liver and peripheral tissues. Human GH can also be detected in urine, a finding that could be helpful in diagnosing certain GH deficiencies.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target||Growth Hormone|
|Assay Target Class||Hormone|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA human growth hormone AL214||PDF 210 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|