The AlphaLISA® Human Plasma Fibronectin Detection Kit is designed for detection and quantitation of human plasma fibronectin (hFN) in human serum, plasma, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL351HV||100 assay points||433.00 USD|
|AL351C||500 assay points||1433.00 USD|
|AL351F||5,000 assay points||9500.00 USD|
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Fibronectin is a large glycoprotein of the extracellular matrix that exists in two forms: insoluble (cellular) and as a soluble plasma protein. The primary functions of fibronectin are its involvement in cell adhesion, growth, migration, and differentiation. Fibronectin is known to contribute to the wound healing process where it will accumulate at the site of injury to help form blood clots and aid in the development of new tissues. Fibronectin levels in plasma have been identified to increase significantly after major traumas. Further, Fibronectin levels have been observed to be significantly altered in disease states of certain types of cancer and also diabetes mellitus. This AlphaLISA kit has been designed to detect and quantify Human Plasma Fibronectin in human serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 124 KB|
|Data Sheet||AlphaLISA human plasma Fibronectin Detection Kit||PDF 535 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|