The AlphaLISA® Human Erythropoietin (EPO) Detection Kit is designed for detection and quantitation of human EPO in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL206C||500 assay points||2827.00 USD|
|AL206F||5,000 assay points||22100.00 USD|
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Human Erythropoietin hormone (EPO) is a 34 kDa glycoprotein produced by kidney cells which are sensitive to oxygen levels in the blood and release EPO upon decreased of oxygen levels in the blood. EPO in the bone marrow then stimulates differentiation and development of red blood cells and initiates the production of haemoglobin thereby increasing the oxygen-carrying capacity of the blood. Polycythemia, a disorder in which there is an excess of red blood cells has been associated with elevated levels of EPO whereas lower than normal levels of EPO is used in chronic renal failure detection.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Central Nervous System|
|Unit Size||5,000 assay points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Data Sheet||Manual AlphaLISA Human Erthyropoietin (EPO) AL206||PDF 212 KB|
|Poster||A Comparison of AlphaLISABead-Based Luminescence and Electrochemiluminescence Immunoassay Technologies for Detection of Human EPO, Amyloid Beta 42 and VEGF in Complex Sample Matrices||PDF 179 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|
|Flyer||AlphaLISA - Frequently Asked Questions||PDF 102 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|